Evaluation of GNMT Gene Expression in Prostate Cancer Tissues using Real-Time PCR
Authors
Abstract:
Introduction: Prostate cancer is the second most common cancer and the leading cause of cancer-related deaths worldwide. In the present study, the expression level of glycine N-methyl transferase gene (GNMT) was investigated in prostate cancer tissue. The GNMT enzyme is encoded by the GNMT gene. Increased GNMT gene expression increases the conversion of glycine to sarcosine and results in the elevated levels of sarcosine in blood and urine. Methods: The expression level of GNMT gene in tissue samples of patients with prostate cancer was compared with those with benign prostatic hyperplasia using Real-Time PCR technique. Results: The GNMT gene expression level increased significantly in prostate cancer patients compared with those with benign prostatic hyperplasia (p-value <0.001). In addition, the expression level of GNMT gene was stage-dependent and significant increases were observed in all stages of prostate cancer compared with those with benign prostatic hyperplasia (p-value <0.001). Conclusion: The concentration of sarcosine is controlled by GNMT and it seems that increasing the expression level of GNMT gene increases the level of sarcosine concentration. Thus, it appears that increased levels of GNMT expression occur in the early stages of prostate cancer. Therefore, periodic measurement of GNMT expression levels can detect prostate cancer before it forms a cancer cell and invades other tissues.
similar resources
Evaluation of gene expression level of GPRC6A, E.cadherin and ZEB1 in prostate cancer in comparison to benign tissues
Background: Prostate cancer is currently the third malignant disease in Iran and fifth common cancer worldwide. The aim of this study was to determine the expression of GPRC6A, E.cadherin, and ZEB1 genes in prostate cancer in comparison with benign tumor. Since early detection of cancer plays an important role in treatment, this study aims to identify the role of GPRC6A, E.cadherin and ZEB1 gen...
full textStudy of the role of HOTAIR gene on colorectal cancer using Real-time PCR
BBackground: Despite advances in cancer studies, colorectal cancer, as the third most common cancer, has the highest mortality rate worldwide. Due to its high prevalence in the younger ages and advanced stages, screening of this cancer with molecular methods is necessary. Studies have shown that HOTAIR gene plays an important role in cancers. Our aim in the present study was to determine the...
full textComparison of seminal vesicle, non-malignant and malignant prostate tissues with gene expression patterns using quantitative real-time PCR.
full text
Investigation of marA Efflux Pump Gene Expression in Ciprofloxacin Resistant Salmonella enteritidis Clinical Strains Using Real-Time PCR
Background and Aim: Ciprofloxacin-resistant Salmonella spp. are among the most important causative agents of food-borne infections. The marA efflux pump in this bacterium plays a significant role in the development of drug resistance. The aim of this study was to investigate marA efflux pump gene expression in Ciprofloxacin resistant Salmonella enteritidis clinical strains by Real Time PCR. Mat...
full textExpression Analysis of Interferon Beta Level in HEK293T Cells Using Real-Time PCR and Protein Tests
Background: Interferons are some kind of natural cytokines which express in response to a variety of antigens including viral RNA, bacterial products, and tumor proteins. Interferon beta is used in the treatment of autoimmune diseases such as multiple sclerosis. Moreover, this drug inhibits cellular proliferation as well as angiogenesis and as a result, helps to cure cancer. In this research, i...
full textDetermination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR
Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...
full textMy Resources
Journal title
volume 19 issue 5
pages 44- 54
publication date 2021-01
By following a journal you will be notified via email when a new issue of this journal is published.
No Keywords
Hosted on Doprax cloud platform doprax.com
copyright © 2015-2023